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Use of molecular hybridization to detect type D retrovirus markers in rhesus placentas and other tissues
Abstract:
We have shown previously that approximately 20% of the Mason-Pfizer virus (MPV) genome is present as endogenous provirus in rhesus monkeys. We report here that several full-term rhesus placentas examined contain additional MPV proviral sequences in their DNA. Competitive molecular hybridization experiments demonstrated that some of these placentas also contain RNA complementary to the entire MPV 60 to 70S RNA genome. Examination of internal organs of rhesus monkeys captured in the wild also revealed the presence of additional MPV proviral sequences and expression of MPV RNA in some tissues. These results provide further evidence that MPV is being transmitted via a non-germ line mechanism in the rhesus population and now demonstrate the placenta as a good source for the identification of retrovirus transcriptional products and proviral DNA.
Insights
Mason-Pfizer virus (MPV) proviral DNA and RNA were found in rhesus monkey placentas and some wild-caught monkey tissues. This suggests non-germ line transmission and highlights the placenta for retrovirus research.
Area of Science:
- Virology
- Genetics
- Primate Biology
Background:
- Endogenous retroviruses can integrate into host genomes.
- Previous studies identified Mason-Pfizer virus (MPV) proviral DNA in approximately 20% of rhesus monkeys.
Purpose of the Study:
- To investigate the presence and expression of MPV in rhesus monkey placentas and wild-caught tissues.
- To further understand the transmission mechanisms of MPV in rhesus populations.
Main Methods:
- Competitive molecular hybridization was used to detect MPV RNA and DNA.
- Analysis of DNA from full-term rhesus placentas.
- Examination of internal organs from wild-caught rhesus monkeys.
Main Results:
- Additional MPV proviral sequences were detected in rhesus monkey placentas.
- Some placentas contained RNA complementary to the full MPV genome.
- MPV proviral sequences and RNA expression were found in some tissues of wild-caught rhesus monkeys.
Conclusions:
- These findings provide evidence for non-germ line transmission of MPV in rhesus monkeys.
- Rhesus monkey placentas are a valuable source for identifying retroviral transcriptional products and proviral DNA.