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New Ultraviolet spectrophotometric method for the estimation of nimesulide
S Chandran1, S Saggar, K P Priya
1Department of Pharmacy, Birla Institute of Technology and Science, Pilani, Rajasthan, India.
Drug Development and Industrial Pharmacy
|March 4, 2000
Summary
Two simple UV spectrophotometric methods accurately estimate nimesulide in pure and solid forms using acetonitrile solvents. These methods offer a wide detection range and high precision for pharmaceutical analysis.
Area of Science:
- Analytical Chemistry
- Pharmaceutical Analysis
Background:
- Nimesulide is a widely used non-steroidal anti-inflammatory drug (NSAID).
- Accurate quantification of nimesulide in pharmaceutical formulations is crucial for quality control.
Purpose of the Study:
- To develop and validate two simple, accurate UV spectrophotometric methods for nimesulide estimation.
- To evaluate the methods' performance in different acetonitrile solvent systems (50% v/v and 100% v/v).
Main Methods:
- UV spectrophotometry was employed for quantitative analysis.
- Linear regression analysis and error propagation theory were used to determine method parameters.
- Method validation included linearity, detection limits, precision, accuracy, and recovery studies.
Main Results:
- Both methods demonstrated linearity in the range of 10-50 µg/ml at 300 nm.
- Detection limits were found to be 0.46 µg/ml (50% acetonitrile) and 1.04 µg/ml (100% acetonitrile).
- The methods showed high precision and accuracy when applied to commercial nimesulide tablet formulations.
Conclusions:
- Simple and accurate UV spectrophotometric methods for nimesulide quantification were successfully developed.
- The developed methods are suitable for routine quality control of nimesulide in pure and solid dosage forms.
- The use of different acetonitrile concentrations provides flexibility in analytical procedures.