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Updated: Aug 10, 2026

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Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
Published on: January 3, 2011
The development of and perspectives for genetic engineering of malaria parasites
1Department of Parasitology, Leiden University Medical Centre, The Netherlands. a.p.waters@parasitology.medfac.leidenuniv.nl
Abstract:
The genetic manipulation of malaria parasites is a rapidly emerging technology that offers great promise for the investigation of many aspects of infection. Currently it is possible to transform avian, rodent, primate as well as human parasites, the latter three on a stable, drug selectable basis. This review focuses on the history of the development of the technology, current abilities and future perspectives.
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Malaria pathogenesis in humans reflects a delicate interplay between parasite biology and host response. Clinical illness reflects a host’s immune response to the parasite’s asexual replication cycle, which is often asymptomatic in individuals with partial immunity. From the parasite's perspective, transmission between mosquito and human with minimal host pathology is evolutionarily advantageous. Among the six Plasmodium species infecting humans, P. falciparum and P. vivax dominate in global...

