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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
Published on: March 17, 2013
Nonlymphoid intraepidermal mononuclear cell collections (pseudo-Pautrier abscesses): a morphologic and
E Candiago1, D Marocolo, M A Manganoni
1Department of Pathology, University of Brescia, Italy.
The American Journal of Dermatopathology
|March 4, 2000
Summary
Intraepidermal collections of mononuclear cells (ICMC) are common in inflammatory skin conditions like spongiotic dermatitis. These cells, resembling dendritic cells, originate from monocytes and differentiate into Langerhans cells.
Area of Science:
- Dermatopathology
- Immunohistochemistry
- Cell Biology
Background:
- Intraepidermal collections of mononuclear cells (ICMC) are observed in various skin conditions.
- Their precise nature and origin in inflammatory dermatoses and lymphomas are not fully understood.
Purpose of the Study:
- To investigate the incidence, morphology, and immunophenotype of ICMC.
- To determine the association of ICMC with specific inflammatory dermatoses and cutaneous lymphomas.
Main Methods:
- Analysis of 1,248 skin biopsies from patients with inflammatory dermatoses and cutaneous lymphomas.
- Morphological assessment and immunohistochemical staining for various cell markers (CD1a, S-100, CD14, CD11b, CD36, CD68, CD83).
Main Results:
- ICMC were identified in 9.9% of biopsies, notably frequent in spongiotic dermatitis (43.4%) and lichenoid dermatitis (10%).
- Cells within ICMC expressed markers of mature Langerhans cells (CD1a, S-100) and monocyte/macrophage lineage (CD11b, CD36, CD68), with some expressing CD83.
- ICMC were also found in cutaneous T-cell lymphoma, mimicking Pautrier abscesses.
Conclusions:
- ICMC represent a frequent, yet understudied, phenomenon in dermatopathology.
- These collections are composed of dendritic cells and their precursors, likely derived from circulating monocytes and differentiating into Langerhans cells.
- ICMC are particularly prevalent in spongiosis-associated dermatoses and can be mistaken for Pautrier abscesses in cutaneous T-cell lymphoma.
