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Anticoagulative effect of pepsin.

M Gacko1, W Roszkowska-Jakimiec, J Jasielczuk

  • 1Department of Instrumental Analysis, Medical Academy, Białystok, Poland.

Acta Biochimica Polonica
|March 4, 2000
PubMed
Summary

Pepsin exhibits anticoagulative properties at concentrations of 36 microM and above by inhibiting fibrin polymerization. This effect is reversed by protamine and does not impact platelet aggregation.

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Area of Science:

  • Biochemistry
  • Hematology

Background:

  • Pepsin is a digestive enzyme.
  • Its potential role in blood coagulation is not well-established.

Purpose of the Study:

  • To investigate the anticoagulative effect of pepsin in vitro.
  • To elucidate the mechanism of pepsin's anticoagulant activity.

Main Methods:

  • In vitro assessment of pepsin's effect on blood coagulation parameters.
  • Evaluation of pepsin's impact on fibrin monomer polymerization.
  • Testing the effect of protamine on pepsin-induced anticoagulation.
  • Assessing pepsin's influence on platelet aggregation induced by ADP and collagen.

Main Results:

  • Pepsin demonstrated anticoagulative effects at concentrations of 36 microM and higher.
  • The anticoagulative action of pepsin was attributed to the inhibition of fibrin monomer polymerization.
  • Protamine was found to abolish the anticoagulative effect of pepsin.
  • Pepsin did not affect platelet aggregation induced by either ADP or collagen.

Conclusions:

  • Pepsin possesses in vitro anticoagulative properties mediated by the inhibition of fibrin polymerization.
  • Protamine can reverse the anticoagulative effect of pepsin.
  • Pepsin does not interfere with platelet aggregation pathways involving ADP or collagen.

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