Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amyloid B-Protein Aggregation at Physiologically Relevant Concentrations. A Critical Role of Membranes.

Alzheimer's research & therapy open access·2022
Same author

Interaction of Amyloidogenic Proteins with Membranes and Molecular Mechanism for the Development of Alzheimer's disease.

Alzheimer's research & therapy open access·2020
Same author

A positive bacterial culture from allograft bone at implantation does not correlate with subsequent surgical site infection.

The bone & joint journal·2015
Same author

Thermodynamics and structure of actinide(IV) complexes with nitrilotriacetic acid.

Inorganic chemistry·2009
Same author

Two-step hard X-ray focusing combining Fresnel zone plate and single-bounce ellipsoidal capillary.

Journal of synchrotron radiation·2007
Same author

Submicrometer hard X-ray focusing using a single-bounce ellipsoidal capillary combined with a Fresnel zone plate.

Journal of synchrotron radiation·2007

Related Experiment Video

Updated: Jul 26, 2026

CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

A cruciform structural transition provides a molecular switch for chromosome structure and dynamics.

L S Shlyakhtenko1, P Hsieh, M Grigoriev

  • 1Department of Microbiology, Arizona State University, Tempe, AZ 85287-2701, USA.

Journal of Molecular Biology
|March 4, 2000
PubMed
Summary

DNA cruciform structures act as molecular switches, regulating genetic processes. Their structural transitions control communication between distant DNA regions, impacting replication and gene expression.

More Related Videos

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
09:13

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Related Experiment Videos

Last Updated: Jul 26, 2026

CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion
09:17

Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion

Published on: March 1, 2022

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
09:13

Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

Area of Science:

  • Molecular Biology
  • Genetics
  • Biophysics

Background:

  • Specific DNA site interactions are vital for genetic regulation.
  • Mechanisms governing these DNA interactions remain largely unknown.
  • Cruciform structures are known to exist in vivo and play roles in replication initiation and gene expression regulation.

Purpose of the Study:

  • To investigate the role of DNA cruciform structural transitions in regulating interactions between distant DNA sites.
  • To elucidate the mechanism by which cruciform conformations influence DNA communication.

Main Methods:

  • Utilized atomic force microscopy (AFM) to visualize and analyze DNA structures.
  • Observed structural transitions between different cruciform conformations.

Main Results:

  • Demonstrated that structural transitions of DNA cruciforms function as a molecular switch.
  • Showed that these transitions can either facilitate or inhibit communication between distant DNA regions.
  • Provided evidence for the dynamic nature of cruciform structures in regulating DNA interactions.

Conclusions:

  • Structural transitions of DNA cruciforms are a key regulatory mechanism.
  • These transitions play a critical role in controlling DNA site communication.
  • The findings suggest a significant role for cruciform structural dynamics in fundamental genetic processes like replication and gene expression.