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Published on: July 18, 2011
Salmonella pathogenicity island 2-dependent evasion of the phagocyte NADPH oxidase
A Vazquez-Torres1, Y Xu, J Jones-Carson
1Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
Abstract:
A type III protein secretion system encoded by Salmonella pathogenicity island 2 (SPI2) has been found to be required for virulence and survival within macrophages. Here, SPI2 was shown to allow Salmonella typhimurium to avoid NADPH oxidase-dependent killing by macrophages. The ability of SPI2-mutant bacteria to survive in macrophages and to cause lethal infection in mice was restored by abrogation of the NADPH oxidase-dependent respiratory burst. Ultrastructural and immunofluorescence microscopy demonstrated efficient localization of the NADPH oxidase in the proximity of vacuoles containing SPI2-mutant but not wild-type bacteria, suggesting that SPI2 interferes with trafficking of oxidase-containing vesicles to the phagosome.
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