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Related Experiment Videos

Bacterial reductive dissolution of crystalline Fe(III) oxide in continuous-flow column reactors.

E E Roden1, M M Urrutia, C J Mann

  • 1Department of Biological Sciences, The University of Alabama, Tuscaloosa, Alabama 35487-0206, USA. eroden@biology.as.ua.edu

Applied and Environmental Microbiology
|March 4, 2000
PubMed
Summary

Continuous-flow reactors enabled near-quantitative bacterial reduction of iron (Fe(III)) oxide-coated sand, unlike batch cultures. Removing dissolved iron (Fe(II)) sustained bacterial activity and growth, impacting contaminant fate.

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Area of Science:

  • Geochemistry
  • Environmental Microbiology
  • Biogeochemical Cycles

Background:

  • Bacterial reduction of iron (Fe(III)) oxides is crucial in geochemical processes.
  • Surface-bound Fe(II) can passivate Fe(III) oxide reduction activity.
  • Understanding Fe(III) oxide reduction is key for contaminant fate studies.

Purpose of the Study:

  • To compare bacterial Fe(III) oxide reduction in continuous-flow column reactors versus batch cultures.
  • To investigate the role of Fe(II) removal in sustaining microbial activity.
  • To assess the implications for contaminant geochemistry.

Main Methods:

  • Studied bacterial reductive dissolution of synthetic Fe(III) oxide-coated sand.
  • Utilized continuous-flow column reactors and parallel batch cultures.

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  • Analyzed aqueous Fe(II) export and remaining Fe(III) content via wet-chemical analysis.

    • Main Results:

    • Column reactors achieved near-quantitative Fe(III) reduction (95.0 +/- 3.7%) over 6 months.
    • Batch cultures showed limited Fe(III) reduction (13.0 +/- 2.2%) and reached an asymptote.
    • Continuous flow sustained bacterial growth (100-fold increase) by removing passivating Fe(II).

    Conclusions:

    • Aqueous-phase transport of Fe(II) in flow reactors prevents passivation, enhancing Fe(III) oxide reduction.
    • Sustained reduction and bacterial growth are achievable in flow systems.
    • Findings inform the geochemical behavior of contaminants linked to Fe(III) reduction.