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A flow cytometry method for rapid detection and enumeration of total bacteria in milk
T S Gunasekera1, P V Attfield, D A Veal
1Centre for Fluorimetric Applications in Biotechnology, Department of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia. tgunasek@rna.bio.mq.edu.au
Applied and Environmental Microbiology
|March 4, 2000
Abstract:
Application of flow cytometry (FCM) to microbial analysis of milk is hampered by the presence of milk proteins and lipid particles. Here we report on the development of a rapid (/= 0.98) between the FCM assay and the more conventional methods of plating and direct microscopic counting was achieved. Raw milk data showed a significant correlation (P < 0.01) and a good agreement (r = 0.91) between FCM and standard plate count methods. The detection limit of the FCM assay was