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Related Experiment Videos

Dishevelled phosphorylation, subcellular localization and multimerization regulate its role in early embryogenesis.

U Rothbächer1, M N Laurent, M A Deardorff

  • 1Division of Biology and Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA. rothbach@lgpd.univ-mrs.fr

The EMBO Journal
|March 4, 2000
PubMed
Summary

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Dishevelled (Dsh) protein is key for Xenopus dorsal-ventral patterning, undergoing post-translational modification on the dorsal side. Its DIX and N-terminal DEP domains are crucial for axis induction, not just membrane translocation.

Area of Science:

  • Developmental biology
  • Molecular and cellular biology
  • Xenopus embryology

Background:

  • Dishevelled (Dsh) is implicated in secondary axis formation and membrane translocation upon Frizzled activation.
  • Previous studies using dominant-negative approaches did not support a role for Dsh in primary axis formation.

Purpose of the Study:

  • To investigate the role of Dishevelled (Dsh) in Xenopus dorsal-ventral patterning.
  • To analyze the influence of endogenous Frizzleds and Dsh domain dependency on its functions.
  • To establish functional links between Dsh modification, membrane translocation, and axis induction.

Main Methods:

  • Analysis of Dishevelled (Dsh) protein post-translational modification in Xenopus embryos.
  • Investigating the effects of endogenous Frizzleds on Xenopus Dsh (Xdsh) translocation and phosphorylation.

Related Experiment Videos

  • Dsh deletion analysis to determine domain requirements for axis induction and membrane association.

    • Main Results:

    • Dsh undergoes post-translational modification at the dorsal side of Xenopus embryos, suggesting a role in dorsal-ventral patterning.
    • Xenopus Frizzleds phosphorylate and translocate Xdsh to the membrane, but this is insufficient for ectopic axis induction.
    • Axis induction ability did not correlate with membrane association; the DIX domain and N-terminal DEP domain are necessary for axis induction, while the DEP domain mediates membrane association and phosphorylation.
    • Dsh forms homomeric complexes in embryos, indicating the importance of multimerization for its function.

    Conclusions:

    • Dsh plays a critical role in Xenopus dorsal-ventral patterning through post-translational modifications.
    • Membrane translocation of Dsh is not sufficient for axis induction; specific domains (DIX and N-terminal DEP) are essential.
    • Dsh multimerization is likely important for its proper embryonic function.