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Kinetics of cassava starch hydrolysis with Termamyl(R) enzyme
D Paolucci-Jeanjean1, M P Belleville, N Zakhia
1LMPM (UMR 5635), ENSCM, 276 Rue de la Galéra, 34097 Montpellier Cedex, France.
Abstract:
Kinetic properties of Termamyl(R) 120L were investigated with respect to starch hydrolysis in a batch reactor at substrate concentrations of 50-270 g. dm(-3) and enzyme concentrations of 0. 17-1 cm(3). dm(-3) (i.e., cm(3) of Novo Nordisk enzymatic solution per dm(3) of raw cassava starch suspension). A general kinetic expression giving product concentration as a function of time was first developed; an equation relating the reaction rate of each product to the sum of the concentrations of oligosaccharides with a higher degree of polymerisation was then derived. The empirical model satisfactorily fits experimental data for oligosaccharides with a degree of polymerization ranging from 1 to 7.
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