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Role of substance P in central control of ovulation in female rats
1Department of Physiology, Institute of Physiology and Biochemistry, Medical University of Lodz, 90-131 Lodz, Poland.
Objective:
To investigate the influence of substance P on gonadotropin release from the pituitary as evaluated according to the final effect, i.e. the ovulation.
Methods:
Stainless steel tube was implanted into the 3rd cerebral ventricle and substance P (SP), C-terminal hexapeptide of Substance P (SP6-11), Gn-RH and adrenaline were infused on the 3rd day of the cycle (proestrus). The oviducts were then isolated on the day of estrus and the ova were counted.
Results:
SP or SP6-11 inhibited the ovulation which was not prevented by intracerebroventricular (i.c.v.) capsaicin pretreatment. The i.c. v. administration of noradrenaline on the 7-8th day of pseudopregnancy induced the ovulation which was prevented by the injection of SP immediately before noradrenaline.
Conclusions:
It is suggested that noradrenergic neuros which control the ovulation by influencing the release of Gn-RH into pituitary portal vessels are affected by SP-ergic neurons.