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Related Experiment Videos

Structural genomics and its importance for gene function analysis.

J Skolnick1, J S Fetrow, A Kolinski

  • 1Laboratory of Computational Genomics, The Danforth Plant Science Center, 893 N, Warson Rd., St. Louis, MO 63141, USA. skolnick@danforthcenter.org

Nature Biotechnology
|March 4, 2000
PubMed
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Structural genomics determines protein structures first, then function. Scanning structures for active site matches offers a new way to assign protein function, even with predicted structures.

Area of Science:

  • Structural biology
  • Genomics
  • Biochemistry

Background:

  • Structural genomics aims to determine all protein fold structures.
  • This shifts focus from known proteins to determining structure before function.
  • Traditional methods rely on sequence motifs for function assignment.

Purpose of the Study:

  • To explore structure-based methods for protein function assignment.
  • To evaluate the use of predicted protein structures for function assignment.

Main Methods:

  • Comparing protein structures to known active sites based on geometry and chemical identity.
  • Utilizing low-resolution structures from contemporary structure prediction methods.

Main Results:

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  • Protein function can be assigned by matching structural features to known active sites.
  • This approach is effective even with low-resolution, predicted structures.

    • Conclusions:

    • Structure-based function assignment is a viable alternative to sequence-based methods.
    • Structural genomics can leverage predicted structures for high-throughput function assignment.