Related Experiment Videos
API2-MALT1 chimeric transcripts involved in mucosa-associated lymphoid tissue type lymphoma predict heterogeneous
M Motegi1, M Yonezumi, H Suzuki
1Laboratory of Chemotherapy, Aichi Cancer Center Research Institute, Aichi Cancer Center Hospital, Nagoya, Japan.
The American Journal of Pathology
|March 7, 2000
Summary
A new RT-PCR assay detects API2-MALT1 fusion transcripts in mucosa-associated lymphoid tissue (MALT) lymphoma. This molecular tool aids in understanding MALT lymphoma pathogenesis and clinical significance.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Low-grade B cell lymphoma molecular analysis identified API2 and MALT1 genes involved in t(11;18)(q21;q21) chromosome aberration.
- This aberration is characteristic of mucosa-associated lymphoid tissue (MALT) type lymphoma.
Purpose of the Study:
- To establish and utilize a reverse transcription-polymerase chain reaction (RT-PCR) assay for detecting API2-MALT1 chimeric transcripts in MALT lymphoma.
- To analyze the presence and characteristics of API2-MALT1 fusion transcripts in a series of MALT lymphoma cases.
Main Methods:
- Development of a reverse transcription-polymerase chain reaction (RT-PCR) assay.
- Analysis of 22 MALT lymphoma cases using the established RT-PCR assay.
- Sequencing analysis of amplified fusion transcripts to identify breakpoints and predict chimeric proteins.
Main Results:
- The RT-PCR assay successfully detected API2-MALT1 chimeric transcripts in all five cases with known t(11;18)(q21;q21).
- Three additional cases without cytogenetic data showed the presence of fusion transcripts, suggesting a higher prevalence of t(11;18) in MALT lymphoma.
- Sequencing revealed multiple breakpoints in API2 and MALT1, resulting in in-frame fusion transcripts and the potential for four different chimeric proteins.
Conclusions:
- The developed RT-PCR assay is an effective molecular tool for identifying API2-MALT1 fusions in MALT lymphoma.
- This assay aids in understanding the molecular pathogenesis and clinical significance of API2-MALT1 rearrangements in MALT lymphomas.
- The findings indicate a significant percentage of MALT lymphoma cases harbor the t(11;18) translocation, detectable via API2-MALT1 fusion transcripts.