Related Experiment Video

Updated: Aug 10, 2026

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1
07:32

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

Published on: September 5, 2016

Phagocytic function of monocytes in children with human immunodeficiency virus type 1 infection

G Tardei1, D Duiculescu, C Capo

  • 1St. S. Nicolau Institute of Virology, Bucharest, Romania. gtardei@hotmail.com

Clinical and Diagnostic Laboratory Immunology
|March 7, 2000
PubMed

Insights

Monocyte phagocytic function, specifically CR3-mediated phagocytosis, was elevated in children with HIV-1 and tuberculosis. This finding was independent of CD4 counts and p24 antigen levels, offering new insights into HIV-1 immune responses.

Area of Science:

  • Immunology
  • Virology
  • Pediatrics

Background:

  • Human immunodeficiency virus type 1 (HIV-1) infection impacts immune cell function in children.
  • Pulmonary tuberculosis is a common opportunistic infection in individuals with HIV-1.
  • Monocytes play a crucial role in innate immunity and pathogen clearance.

Purpose of the Study:

  • To compare the phagocytic function of monocytes in children with HIV-1 and pulmonary tuberculosis to healthy controls.
  • To investigate the role of CR3-mediated phagocytosis in this pediatric cohort.
  • To determine if CD4 counts and p24 antigenemia influence phagocytic function.

Main Methods:

  • Monocyte phagocytosis assay.
  • Flow cytometry to assess CR3 expression and function.
  • Comparison between HIV-1 infected children with tuberculosis and age-matched healthy controls.

Main Results:

  • CR3-mediated phagocytosis was significantly increased in children with HIV-1 and pulmonary tuberculosis.
  • This enhancement in phagocytosis was observed irrespective of CD4 T-cell counts.
  • Elevated phagocytosis was also independent of detectable p24 antigen levels.

Conclusions:

  • Children with HIV-1 and co-existing pulmonary tuberculosis exhibit augmented CR3-mediated phagocytic capacity.
  • This suggests a potential compensatory immune mechanism or disease-specific alteration in monocyte function.
  • Further research is warranted to explore the clinical implications of these findings in pediatric HIV-1 management.

More Related Videos

Isolation, Transfection, and Culture of Primary Human Monocytes09:13

Isolation, Transfection, and Culture of Primary Human Monocytes

Published on: December 16, 2019

Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets08:12

Isolation of Leukocytes from Human Breast Milk for Use in an Antibody-dependent Cellular Phagocytosis Assay of HIV Targets

Published on: September 6, 2019

Related Concept Videos

Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
Immune Surveillance by NK Cells and Phagocytes01:25

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...