Related Experiment Videos
Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken
D K Griffin1, F Haberman, J Masabanda
1Department of Biological Sciences, Brunel University, Uxbridge, Middlesex, UK. Darren.Griffin@brunel.ac.uk
Cytogenetics and Cell Genetics
|March 7, 2000
Abstract:
Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes.