Jove
Visualize
Contact Us

Related Experiment Videos

Automated solid-phase extraction workstations combined with quantitative bioanalytical LC/MS.

N H Huang1, J R Kagel, D T Rossi

  • 1Department of Pharmacokinetics and Dynamics, Metabolism, Parke-Davis Pharmaceutical Research, Division of Warner-Lambert, Ann Arbor, MI 48105, USA.

Journal of Pharmaceutical and Biomedical Analysis
|March 7, 2000
PubMed
Summary

An automated method for quantifying a novel lipid-regulating drug in dog plasma was developed using solid-phase extraction and LC/MS/MS. This efficient approach offers high accuracy and recovery, enabling rapid sample analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Association between genetic predisposition to childhood obesity and the risk of adult ischemic heart disease in China].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2022
Same author

[Development and outlook on human challenge trial of vaccine].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2021
Same author

[Hesitancy of parents towards vaccines in national immunization program in three regions in China: a cross-sectional study].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2021
Same author

[Studies on sexual and childbearing-related stigma against chronic hepatitis B patients in four provinces in China].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2020
Same author

LC/MS/MS quantitation of an anti-cancer drug in human plasma using a solid-phase extraction workstation: application to population pharmacokinetics.

Journal of pharmaceutical and biomedical analysis·2001
Same author

Microdialysis sampling of the isothiazolone, PD-161374, and its thiol and disulfide metabolites.

Journal of pharmaceutical and biomedical analysis·2000
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Analytical Chemistry
  • Pharmacology
  • Biotechnology

Background:

  • Accurate quantification of novel drugs in biological matrices is crucial for pharmacokinetic studies.
  • Traditional sample preparation methods can be time-consuming and labor-intensive.

Purpose of the Study:

  • To develop, characterize, and validate an automated LC/MS/MS method for quantifying a novel lipid-regulating drug in dog plasma.
  • To optimize solid-phase extraction (SPE) parameters for efficient sample preparation.

Main Methods:

  • Utilized an automated solid-phase extraction workstation for method development and validation.
  • Employed liquid chromatography-tandem mass spectrometry (LC/MS/MS) with multiple reaction monitoring (MRM) for drug quantification.
  • Investigated various wash solvents, elution solvents, and sorbents to optimize recovery.

Related Experiment Videos

Main Results:

  • Achieved high precision (within 9.8% RSD) and accuracy (-7.2% to +6.2% RE) for drug quantification in dog plasma.
  • Optimized SPE conditions yielded recoveries generally greater than 95%.
  • Demonstrated high sample throughput, with approximately 8 minutes per sample and 100 samples processed in under 120 minutes.

Conclusions:

  • The developed automated SPE-LC/MS/MS method is accurate, precise, and efficient for quantifying a novel lipid-regulating drug in dog plasma.
  • This automated approach significantly enhances sample throughput, making it suitable for high-volume bioanalysis.
  • The method provides a robust platform for drug development and pharmacokinetic studies.