Simultaneous determination of some multicomponent dosage forms by quantitative thin layer chromatography...

Area of Science:

• Analytical Chemistry
• Pharmaceutical Analysis
• Chromatography

Background:

• Accurate quantification of active pharmaceutical ingredients in complex mixtures is crucial for quality control.
• Existing analytical methods may lack the efficiency or specificity for simultaneous determination of multiple drugs.

Purpose of the Study:

• To develop and validate a thin layer chromatography densitometric method for the simultaneous determination of three distinct multicomponent drug mixtures.
• To assess the method's applicability in analyzing pharmaceutical formulations.

Main Methods:

• Thin layer chromatography (TLC) coupled with densitometry was employed.
• Optimized mobile phases (ethyl acetate: methanol: ammonia 25% and ethyl acetate) were used for separating specific drug combinations.
• Retention factor (Rf) values were determined for each analyte.

Main Results:

• The method successfully separated and quantified components in mixtures containing naproxen/diflunisal, paracetamol/chlorzoxazone, and chlorphenoxamine hydrochloride/8-chlorotheophylline/caffeine.
• Linearity, recovery, and precision were evaluated, demonstrating the method's reliability.
• The TLC-densitometric method was successfully applied to pharmaceutical formulations like tablets, capsules, and suppositories.

Conclusions:

• The developed thin layer chromatography densitometric method provides an efficient and accurate approach for the simultaneous analysis of multicomponent pharmaceutical mixtures.
• The method's successful application to various dosage forms validates its utility in pharmaceutical quality control.
• Statistical comparison confirmed the method's efficacy against reported alternative techniques.