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Co-expression of vascular endothelial growth factor and its receptor flt-1 in malignant pleural mesothelioma
J König1, E Tolnay, T Wiethege
1Institute of Pathology, and German Mesothelioma Registry, University Hospital Bergmannsheil, Ruhr University Bochum, Germany.
Background:
Vascular endothelial growth factor (VEGF) is a potent angiogenic protein with a selective mitogenic effect on endothelial cells known to be involved in many normal and pathological processes. Coexpression of VEGF and its receptor flt-1 has been reported in different types of malignant tumors.
Objective:
In the present study we investigated the expression of VEGF and flt-1 in 90 cases of diffuse malignant pleural mesotheliomas.
Methods:
VEGF and flt-1 expression was analyzed by immunohistochemistry and non-radioactive in situ hybridization.
Results:
VEGF expression was visualized immunohistochemically in tumor cells. flt-1 expression correlated with histological differentiation (p < 0.013). Furthermore, expression of flt-1 was detected in tumor cells, macrophages and microvessels adjacent to tumor cells. VEGF and flt-1 expression were confirmed by in situ hybridization.
Conclusion:
There was a statistically significant correlation between VEGF and flt-1 expression (p < 0.001). The observed coexpression of VEGF and flt-1 possibly suggests a potential autocrine loop for malignant pleural mesothelioma cells.