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A mathematical model for yeast respiro-fermentative physiology.

P P Hanegraaf1, A H Stouthamer, S A Kooijman

  • 1Faculty of Biology, Vrije Universiteit Amsterdam, De Boelelaan 1087, 1082 HV Amsterdam, The Netherlands. hgraaf@bio.vu.nl

Yeast (Chichester, England)
|March 8, 2000
PubMed
Summary

This study presents a mechanistic model for yeast respiro-fermentative physiology, explaining how substrate concentration affects glucose uptake and product formation without critical switches.

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Area of Science:

  • Microbiology
  • Biochemical Engineering
  • Systems Biology

Background:

  • Yeast respiro-fermentative physiology is complex, involving multiple glucose uptake and assimilation pathways.
  • Existing models often rely on simplified assumptions like critical switches or maximum respiratory capacity.

Purpose of the Study:

  • To develop a mechanistic model for yeast respiro-fermentative physiology.
  • To explain the relationship between substrate concentration, glucose uptake, and product formation.
  • To provide a generalizable model for heterotrophic microorganism metabolism.

Main Methods:

  • Developed a mechanistic model incorporating multiple glucose carriers and assimilation pathways.
  • Analyzed the mechanistic response of these pathways to varying substrate concentrations.

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  • Estimated model parameters by fitting data from existing literature.

    • Main Results:

    • The model demonstrates how high-affinity/low-rate and low-affinity/high-rate glucose carriers dominate at low and high substrate concentrations, respectively.
    • Lower assimilation efficiency and biomass yield are linked to high uptake rates at high substrate concentrations.
    • Product formation is associated with high uptake rate pathways, explaining observed substrate-product relationships.

    Conclusions:

    • The model successfully explains respiro-fermentative physiology based on carrier kinetics and substrate concentration.
    • It offers a more nuanced understanding than models relying on critical switches.
    • The model's principles are applicable to the general metabolism of heterotrophic microorganisms.