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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
Published on: January 26, 2018
A development-specific histone H3 localizes to the developing macronucleus of Euplotes.
1Department of Biochemistry, University of Connecticut Health Center, Farmington, Connecticut 06032, USA.
Genesis (New York, N.Y. : 2000)
|March 21, 2000
Summary
A variant histone H3(P) protein is highly expressed during macronuclear development in Euplotes crassus. This suggests significant changes in nucleosome composition and chromatin structure are crucial for programmed DNA rearrangement.
Area of Science:
- Molecular Biology
- Cell Biology
- Genetics
Background:
- Euplotes crassus undergoes programmed DNA rearrangement during macronuclear development.
- A gene, H3(P), is known to be expressed during sexual reproduction and encodes a variant histone H3 protein.
Purpose of the Study:
- To generate an antiserum against the H3(P) protein.
- To investigate the expression and localization of H3(P) during macronuclear development.
- To explore changes in nucleosome composition during this process.
Main Methods:
- Antiserum generation against H3(P) protein.
- Immunolocalization studies using the antiserum.
- Analysis of nuclear stages during macronuclear development.
Main Results:
- H3(P) protein shows maximal expression during the polytene chromosome stage.
- H3(P) is localized specifically to the developing macronucleus.
- Evidence suggests the presence of at least one other variant histone in the developing macronucleus.
Conclusions:
- Significant changes in nucleosome composition occur during macronuclear development.
- Altered chromatin structure likely plays a role in the DNA rearrangement processes.
- H3(P) is a key component of the chromatin undergoing dynamic changes.
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