Neuronal loss and apoptosis in experimental Creutzfeldt-Jakob disease in mice

One of the hallmarks of prion disease--neuronal cell loss, may be accomplished by apoptosis. The aim of this study was to estimate the neuronal cell loss in mice brains with experimental Creutzfeldt-Jakob disease (CJD) and control mice in the comparison with the apoptosis appeared by in situ end labelling (TUNEL) in function of time of post-incubation period and developing of the spongiform changes. The number of neurons was considerably lower in terminally sick animals (20-21 week of incubation period) than in control mice. The mean value of loss of neuronal cell was 32%. The greatest loss (55%) of neurons was noted in the septal nuclei of the paraterminal body and the least lost (16%) in the hypothalamus. We report here, that apoptotic cells are readily detectable in CJD-affected mice brains in time-dependt manner after infection of Fujisaki strain, but the number of apoptotic cells detected by in situ end labelling does not well correlate with the extensiveness of neuronal loss. The degree of apoptosis corresponds to the well developed spongiform changes.