Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Candidate genes for the hypoxic tumor phenotype.

A C Koong1, N C Denko, K M Hudson

  • 1Department of Radiation Oncology, Stanford University School of Medicine, California 94305-5468, USA.

Cancer Research
|March 8, 2000
PubMed
Summary

Hypoxia induces genes linked to aggressive cancer phenotypes, including plasminogen activator inhibitor-1 (PAI-1). PAI-1 levels in head and neck cancer patients correlate with tumor hypoxia, suggesting its potential as a biomarker.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting MET and AXL overcomes resistance to sunitinib therapy in renal cell carcinoma.

Oncogene·2015
Same author

A Phase 2 Multi-institutional Study to Evaluate Gemcitabine and Fractionated Stereotactic Radiotherapy for Unresectable, Locally Advanced Pancreatic Adenocarcinoma.

Practical radiation oncology·2014
Same author

CTGF is a therapeutic target for metastatic melanoma.

Oncogene·2013
Same author

A novel epidermal growth factor receptor variant lacking multiple domains directly activates transcription and is overexpressed in tumors.

Oncogene·2011
Same author

Genetically modified clostridium for gene therapy of tumors.

Methods in molecular medicine·2011
Same author

PHD2 in tumour angiogenesis.

British journal of cancer·2010

Area of Science:

  • Molecular biology
  • Cancer research
  • Genomics

Background:

  • Hypoxia, or low oxygen, is a hallmark of solid tumors.
  • Tumor hypoxia is associated with increased aggressiveness and poor prognosis.
  • Identifying genes regulated by hypoxia is crucial for understanding tumor progression.

Purpose of the Study:

  • To identify genes transcriptionally upregulated by hypoxia in squamous cell carcinoma cells.
  • To investigate the potential of these genes as biomarkers for tumor hypoxia and aggressiveness.
  • To analyze the induction kinetics of plasminogen activator inhibitor-1 (PAI-1) under hypoxic conditions.

Main Methods:

  • DNA array analysis to screen for hypoxia-induced genes.
  • Northern blot analysis to confirm gene induction.

Related Experiment Videos

  • Kinetic analysis of PAI-1 mRNA expression under hypoxia and reoxygenation.
  • Measurement of serum PAI-1 levels in cancer patients.

    • Main Results:

    • Identified several hypoxia-induced genes, including PAI-1, IGFBP-3, MMP-13, and VEGF.
    • Confirmed gene induction in squamous cell carcinoma cell lines.
    • Observed a gradual increase in PAI-1 mRNA during hypoxia (2-24h) and rapid decay upon reoxygenation.
    • Found a correlation between serum PAI-1 levels and tumor hypoxia in head and neck cancer patients.

    Conclusions:

    • Hypoxia significantly alters gene expression profiles in cancer cells, promoting an aggressive phenotype.
    • PAI-1 is a hypoxia-responsive gene and a potential serum biomarker for tumor hypoxia in head and neck cancers.