Related Experiment Video
Updated: Aug 11, 2026

Using the Overlay Assay to Qualitatively Measure Bacterial Production of and Sensitivity to Pneumococcal Bacteriocins
Published on: September 30, 2014
[The bacteriocinogenic and lysozyme-synthesizing activity of lactobacilli]
N K Kovalenko1, L N Nemirovskaia, S A Kasumova
1Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Abstract:
It was shown that lactic acid bacteria isolated from different sources produced along with lactic acid the bacteriocine substances. Strains producing bacteriocines were mainly isolated from digestive tract of people and calves. Bacteriogenic properties have been shown more frequently in the case of lactobacilli (7.8%) more rarely--in coccoid forms of lactic acid bacteria (4.5%). At the same time bacteriocine activity was higher in the case of Enterococcus and depended both on the strain type and cultivation conditions. Synthesis of bacteriocines started from the hours of phase of the culture growth and reached maximum at the beginning of stationary phase. The bacteriocine accumulation rate depended on composition of the growth medium.
Related Concept Videos
Operons
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Inducible Operons: lac Operon
Bacterial Phylum Actinobacteria
Microbes in Food Production
Inhibitors of Gram-positive Cell Wall Synthesis

