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Dynamics of beta-glucosidase Zm-p60.1 ectopic expression during transgenic pollen development: a histochemical
J Dubová1, P Mlejnek, B Brzobohatý
1Department of Plant Physiology and Anatomy, Faculty of Sciences, Masaryk University, Brno, Czech Republic. dubova@sci.muni.cz
Abstract:
Zm-p60.1 is maize cDNA coding cytokinin-glucoside specific beta-glucosidase. Indigogenic method was used for histochemical localization of Zm-p60.1 beta-glucosidase activity in various developmental stages of transgenic tobacco anthers. Expression of Zm-p60.1 cDNA in T7 tobacco plants is controlled by the CaMV 35S promoter. Another type of tobacco transformant expresses Zm-p60.1 under the control of LAT 52 promoter. Histochemical detection has proved different patterns of beta-glucosidase activity during tobacco pollen development in these two types of transformants. Zm-p60.1 beta-glucosidase activity had not direct influence on pollen germinability.