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Rabies01:28

Rabies

Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...

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Classical swine fever virus E(rns) deletion mutants: trans-complementation and potential use as nontransmissible,

M N Widjojoatmodjo1, H G van Gennip, A Bouma

  • 1Department of Mammalian Virology, DLO-Institute for Animal Science and Health, Lelystad, The Netherlands.

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|March 9, 2000
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Summary
This summary is machine-generated.

Researchers rescued classical swine fever virus (CSFV) E(rns) deletion mutants using a specialized cell line. Pigs vaccinated with these mutants were protected from CSFV, demonstrating a new vaccine strategy.

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Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Classical swine fever virus (CSFV) is a significant pathogen in swine.
  • The glycoprotein E(rns) plays a crucial role in CSFV infectivity and immune evasion.
  • Developing effective vaccines and diagnostic tools for CSFV is a priority.

Purpose of the Study:

  • To rescue and characterize CSFV E(rns) deletion mutants.
  • To evaluate the potential of these mutants as non-transmissible modified live vaccines.
  • To explore their use in differentiating infected from vaccinated animals.

Main Methods:

  • Construction of CSFV E(rns) deletion mutants (Flc22 and Flc23).
  • Rescue of mutants using a complementing SK6 cell line expressing E(rns).
  • In vitro characterization and in vivo vaccination trials in pigs.

Main Results:

  • E(rns) deletion mutants were successfully rescued in vitro using complementing cells.
  • Rescued viruses could infect and replicate but did not produce infectious progeny.
  • Vaccination with E(rns) deletion mutants conferred protection against lethal CSFV challenge.
  • E(rns) from the complementing cells was incorporated into viral particles.

Conclusions:

  • Demonstrated the first trans-complementation of defective pestivirus RNA with a pestiviral structural protein.
  • Opened new avenues for developing non-transmissible modified live pestivirus vaccines.
  • The absence of the antigenic E(rns) part allows differentiation between infected and vaccinated animals.