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Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
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Published on: January 12, 2018

Erythrocytes stored in CPD SAG-mannitol: evaluation of their deformability

P Izzo1, A Manicone, A Spagnuolo

  • 1Dipartimento di Scienze Biomediche ed Oncologia Umana, Università degli Studi di Bari, Italy.

Clinical Hemorheology and Microcirculation
|March 11, 2000
PubMed

Insights

Erythrocyte deformability, a measure of red blood cell health, can be assessed using the Elongation Index (EI) in stored blood. This method helps evaluate the vitality of erythrocytes in blood samples over time.

Area of Science:

  • Biomedical Engineering
  • Hematology
  • Cell Biology

Background:

  • Erythrocyte deformability is crucial for blood circulation.
  • Stored blood quality is vital for transfusions.
  • Assessing red blood cell vitality is essential for blood banking.

Purpose of the Study:

  • To evaluate erythrocyte deformability in stored blood samples.
  • To explore the Elongation Index (EI) as a measure of erythrocyte vitality.
  • To assess red blood cell health in CPD SAG-mannitol preserved blood.

Main Methods:

  • Utilized Laser-assisted Optical Rotational Cell Analyzer (LORCA).
  • Analyzed three blood samples from healthy donors.
  • Measured progressive reduction in Elongation Index (EI) during storage.

Main Results:

  • Demonstrated a progressive reduction in erythrocyte Elongation Index (EI) over time.
  • Showcased the capability of LORCA in measuring deformability changes.
  • Indicated variability in erythrocyte vitality based on storage duration.

Conclusions:

  • Progressive reduction in EI can serve as an indicator of erythrocyte vitality.
  • LORCA is a suitable tool for evaluating red blood cell deformability in stored blood.
  • The EI offers a quantitative method for assessing the quality of preserved erythrocytes.

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