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Modelisation of leukocyte adhesion on a fibrinogen coated surface in static conditions
V Labrador1, S Legrand, S Muller
1Hémorhéologie-Angiohématologie, UMR CNRS 7563 Fac. Méd., Vandoeuvre, France.
Abstract:
The adhesion of polymorphonuclear leukocytes (PMNs) on the vascular endothelium is a complex process that occurs during biological and pathological events and involves a large family of molecules. This phenomenom could be approached by a modelisation study of the adhesion of PMNs on a biological substrate, fibrinogen. Two different physiological conditions were tested such as the activated state of PMNs with a synthetic pro-inflammatory activator (N-Formyl-Methionyl-Leucyl-Phenylalanine, FMLP). The activated state of PMNs was both quantified by flow cytometry and controlled by fluorescence microscopy. The results suggest that quiescent PMNs deposit in accordance with the ballistic deposition model. The preliminary results obtained with FMLP-stimulated PMNs show a different deposit process compared to quiescent PMNs but do not allow to determine exactly a deposition model.
Insights
Polymorphonuclear leukocytes (PMNs) adhesion to fibrinogen was modeled. Quiescent PMNs followed ballistic deposition, while activated PMNs showed a different, undetermined adhesion process.
Area of Science:
- Cellular Biology
- Biophysics
- Immunology
Background:
- Polymorphonuclear leukocytes (PMNs) adhesion to vascular endothelium is crucial in biological and pathological processes.
- This adhesion involves numerous molecular interactions and can be studied using models.
- Fibrinogen serves as a relevant biological substrate for modeling PMN adhesion.
Purpose of the Study:
- To model the adhesion of PMNs on a fibrinogen substrate.
- To investigate PMN adhesion under quiescent and activated physiological conditions.
- To determine if PMN adhesion follows established deposition models.
Main Methods:
- Utilized flow cytometry and fluorescence microscopy to quantify and control PMN activation.
- Applied a synthetic pro-inflammatory activator, N-Formyl-Methionyl-Leucyl-Phenylalanine (FMLP), to stimulate PMNs.
- Modeled PMN deposition on fibrinogen under different physiological states.
Main Results:
- Quiescent PMNs exhibited deposition patterns consistent with the ballistic deposition model.
- FMLP-stimulated PMNs demonstrated a distinct adhesion process compared to quiescent cells.
- The specific deposition model for activated PMNs could not be precisely determined from preliminary results.
Conclusions:
- The ballistic deposition model effectively describes quiescent PMN adhesion to fibrinogen.
- PMN activation significantly alters their adhesion behavior on fibrinogen.
- Further research is needed to elucidate the precise deposition model for activated PMNs.