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Related Experiment Videos

Purification of lysozyme using ultrafiltration.

R Ghosh1, Z F Cui

  • 1Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, United Kingdom. raja.ghosh@eng.ox.ac.uk

Biotechnology and Bioengineering
|March 11, 2000
PubMed
Summary

This study optimized lysozyme (LYS) separation from chicken egg white using ultrafiltration. Higher pH and transmembrane pressure improved lysozyme purification and flux, with a two-step process showing promise for high purity and productivity.

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Area of Science:

  • Biochemistry
  • Separation Science
  • Food Technology

Background:

  • Lysozyme (LYS) is a valuable antimicrobial enzyme found in egg white.
  • Efficient separation methods are crucial for its industrial applications.
  • Ultrafiltration offers a promising technique for protein purification.

Purpose of the Study:

  • To investigate the ultrafiltration (UF) of lysozyme from chicken egg white.
  • To evaluate the impact of operational parameters on UF performance.
  • To explore a two-step UF process for enhanced lysozyme recovery.

Main Methods:

  • Ultrafiltration using polysulfone membranes with 25 kDa and 50 kDa molecular weight cut-offs (MWCO).
  • Systematic variation of pH, feed concentration, transmembrane pressure, and hydrodynamics.

Related Experiment Videos

  • Analysis of permeate flux, lysozyme transmission, purification factor, and productivity.

    • Main Results:

    • Higher pH increased permeate flux and lysozyme transmission for both membranes.
    • The 25 kDa MWCO membrane yielded higher lysozyme purity.
    • Increased feed concentration reduced lysozyme purity.
    • For the 50 kDa MWCO membrane, higher transmembrane pressure boosted flux, productivity, and purity.
    • A two-step UF process was investigated for improved yield and purity.

    Conclusions:

    • Operational parameters significantly influence lysozyme separation efficiency via UF.
    • Membrane selection (25 kDa vs. 50 kDa MWCO) impacts purity and flux.
    • Optimized conditions and multi-step processes can enhance lysozyme recovery and purity for industrial use.