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Updated: Jul 27, 2026
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Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Genomic-scale gene expression analysis of lymphocyte growth, tolerance and malignancy
R J Glynne1, G Ghandour, C C Goodnow
1Eos Biotechnology, South San Francisco, 94080, USA.
Current Opinion in Immunology
|March 14, 2000
Summary
Immunologists can now monitor numerous gene products simultaneously using gene expression arrays. This technology extends beyond cell surface markers to analyze all gene transcripts within lymphoid cells.
Area of Science:
- Immunology
- Molecular Biology
- Genomics
Background:
- Immunologists routinely monitor multiple gene products, often cell surface markers identified by CD nomenclature.
- Flow cytometry requires increasing numbers of fluorescent colors for multi-parameter analysis.
- Current methods face limitations in comprehensively surveying the full spectrum of cellular gene expression.
Purpose of the Study:
- To introduce gene expression arrays as a powerful tool for immunologists.
- To extend gene expression analysis beyond cell surface markers.
- To enable comprehensive surveying of gene transcripts in lymphoid cells.
Main Methods:
- Application of gene expression arrays.
- Analysis of gene transcripts.
- Focus on lymphoid cell populations.
Main Results:
- Gene expression arrays provide a method for simultaneous monitoring of numerous gene products.
- This approach expands analysis beyond the cell surface.
- Comprehensive surveying of the entire gene transcript catalogue in lymphoid cells is feasible.
Conclusions:
- Gene expression arrays offer a significant advancement for immunological research.
- The technology allows for a more complete understanding of cellular states.
- This method overcomes limitations of traditional multi-parameter analysis.
