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Genetic analysis of SecA-SecY interaction required for spore development in Bacillus subtilis
H Kobayashi1, Y Ohashi, H Nanamiya
1Laboratory of Molecular Genetics, College of Science, Rikkyo (St. Paul's) University, Toshima-ku, Tokyo, Japan.
FEMS Microbiology Letters
|March 14, 2000
Abstract:
All spontaneous suppressor mutations obtained from a secA12 sporulation-defective mutant in Bacillus subtilis were localized in highly conserved membrane-spanning regions of SecY. The expression of early sporulation genes, kinA and spo0A encoding a histidine kinase and a transcription regulator for several sporulation genes, respectively, was restored in these suppressor mutants. These results indicate that the secretion function of translocase combined with Sec proteins is required for sporulation in B. subtilis.