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Published on: March 25, 2009
Cloning and characterization of cDNA encoding zebrafish Danio rerio NM23-B gene
1Institute for Molecular Biology, Seoul National University, Seoul, South Korea. jae-seong.lee@nies.jp
Gene
|March 14, 2000
Abstract:
A full-length zebrafish NM23-B cDNA was cloned and sequenced. The zebrafish NM23-B cDNA consists of 624bp with an open reading frame of 153 amino acids. NM23-B mRNA of approximately 0.7kb is present in adult zebrafish tissues. Zebrafish NM23-B his-tagged protein (17kDa) was produced in E. coli and characterized by binding and UV-cross-linking to a single-stranded telomeric repeat (TTAGGG)(6). This is the first report to show that fish have a NM23-H2 homologue that is similar to that in humans.
