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Crystallization and preliminary X-ray study of beta--mannosidase from Trichoderma reesei
R Aparicio1, E V Eneiskaya, A A Kulminskaya
1National Synchrotron Light Laboratory, LNLS, Caixa Postal 6192, CEP 13083-970, Campinas, SP, Brazil.
Abstract:
beta-Mannosidase from Trichoderma reesei, a 105 kDa glycoprotein, has been crystallized. The crystals belong to the space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell dimensions a = b = 165.86, c = 122.46 A, and diffract beyond 2.75 A resolution. X-ray diffraction data were collected from a frozen crystal on a synchrotron X-ray source.