Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

ATP Synthase: Structure01:18

ATP Synthase: Structure

15.7K
ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
15.7K
ATP Synthase: Mechanism01:48

ATP Synthase: Mechanism

17.3K
In animals, the mitochondrial F1F0 ATP synthase is the key protein that synthesizes ATP molecules through a complex catalytic mechanism. While the nuclear genome encodes the majority of ATP synthase subunits, the mitochondrial genome encodes some of the enzyme's most critical components. The formation of this multi-subunit enzyme is a complex multi-step process regulated at the level of transcription, translation, and assembly. Defects in one or more of these steps can result in decreased...
17.3K
Structures of Solids02:22

Structures of Solids

18.0K
Solids in which the atoms, ions, or molecules are arranged in a definite repeating pattern are known as crystalline solids. Metals and ionic compounds typically form ordered, crystalline solids. A crystalline solid has a precise melting temperature because each atom or molecule of the same type is held in place with the same forces or energy. Amorphous solids or non-crystalline solids (or, sometimes, glasses) which lack an ordered internal structure and are randomly arranged. Substances that...
18.0K
Structural Isomerism02:34

Structural Isomerism

21.7K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula. Structural isomerism of coordination compounds can be divided into two subcategories, the linkage isomers and coordination-sphere isomers.
Linkage isomers occur when the coordination compound contains a ligand that can bind to the transition metal center through two different atoms. For example, the CN− ligand can bind through the carbon atom or through the nitrogen atom. Similarly, SCN− can...
21.7K
Structure of Lipids03:38

Structure of Lipids

99.1K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
99.1K
Additional Subnuclear Structures02:10

Additional Subnuclear Structures

5.4K
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Distinct Mineralogy and Age of Individual Lava Flows in Atla Regio, Venus Derived From Magellan Radar Emissivity.

Journal of geophysical research. Planets·2021
Same author

Directional limits on persistent gravitational waves using LIGO S5 science data.

Physical review letters·2012
Same author

Structural studies of ROK fructokinase YdhR from Bacillus subtilis: insights into substrate binding and fructose specificity.

Journal of molecular biology·2010
Same author

Intermolecular interactions and characterization of the novel factor Xa exosite involved in macromolecular recognition and inhibition: crystal structure of human Gla-domainless factor Xa complexed with the anticoagulant protein NAPc2 from the hematophagous nematode Ancylostoma caninum.

Journal of molecular biology·2006
Same author

What the structure of angiostatin may tell us about its mechanism of action.

Journal of thrombosis and haemostasis : JTH·2004
Same author

Efficacy of nelfinavir in patients switched from ritonavir/saquinavir combination antiretroviral therapy.

HIV clinical trials·2001
Same journal

Structural insights into the synthesis of FMN in prokaryotic organisms.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Serial crystallographic analysis of protein isomorphous replacement data from a mixture of native and derivative microcrystals.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

The first crystal structure of the peptidase domain of the U32 peptidase family.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.

Acta crystallographica. Section D, Biological crystallography·2015
See all related articles

Related Experiment Video

Updated: Feb 10, 2026

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue
13:45

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue

Published on: November 11, 2022

2.6K

Structural studies of MIP synthase.

A J Stein1, J H Geiger

  • 1Michigan State University, Department of Chemistry, East Lansing, MI 48824, USA.

Acta Crystallographica. Section D, Biological Crystallography
|March 14, 2000
PubMed
Summary
This summary is machine-generated.

1-L-myo-inositol 1-phosphate synthase (MIP synthase) is crucial for inositol biosynthesis. Researchers crystallized MIP synthase, revealing its structure for understanding cellular functions like proliferation and signaling.

More Related Videos

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
04:30

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

Published on: January 16, 2020

10.7K
Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
10:39

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Published on: September 14, 2014

31.0K

Related Experiment Videos

Last Updated: Feb 10, 2026

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue
13:45

A Method to Study the Correlation Between Local Collagen Structure and Mechanical Properties of Atherosclerotic Plaque Fibrous Tissue

Published on: November 11, 2022

2.6K
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster
04:30

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

Published on: January 16, 2020

10.7K
Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
10:39

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

Published on: September 14, 2014

31.0K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Cell Biology

Background:

  • 1-L-myo-inositol 1-phosphate synthase (MIP synthase) catalyzes the rate-limiting step in de novo inositol biosynthesis.
  • Inositol and its derivatives are vital for cell membrane structure and signal transduction, impacting cellular functions such as proliferation and secretion.

Purpose of the Study:

  • To elucidate the structure of MIP synthase through X-ray crystallography.
  • To provide insights into the regulation and function of MIP synthase in eukaryotic cells.

Main Methods:

  • Overexpression and purification of MIP synthase in Escherichia coli.
  • X-ray crystallography using hanging-drop vapor-diffusion to obtain two crystal forms.
  • Data collection using a Rigaku R-AXIS IIC imaging-plate detector.

Main Results:

  • Two distinct crystal forms of MIP synthase were obtained.
  • Crystal form I (space group C2) diffracts to 2.5 A resolution.
  • Crystal form II (space group P2(1)) diffracts to 2.9 A resolution.

Conclusions:

  • The structural determination of MIP synthase provides a foundation for understanding its enzymatic mechanism.
  • Insights into MIP synthase structure can inform research on cellular processes regulated by inositol metabolism.