Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

A simple, nondestructive assay for bound hyaluronan.

¹Beacon Research/Biocoat, Inc., P.O. Box 277, Glenside, Pennsylvania 19038, USA.

Journal of Biomedical Materials Research

|March 14, 2000

Summary

A new, non-damaging method quantifies bound hyaluronan using Toluidine Blue O dye. This technique is ideal for hyaluronan-coated medical devices, offering easy application for manufacturers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Rapid improvement in symptoms and physical function following ibrutinib initiation in chronic lymphocytic leukemia and the associated changes in plasma cytokines.

Leukemia research·2021

Same author

Descriptive analysis of dosing and outcomes for patients with ibrutinib-treated relapsed or refractory chronic lymphocytic leukemia in a Canadian centre.

Current oncology (Toronto, Ont.)·2019

Same author

In-vitro stability of porcine factor VIII (Hyate:C(®) ).

Haemophilia : the official journal of the World Federation of Hemophilia·2016

Same author

Lysosomotropic agents selectively target chronic lymphocytic leukemia cells due to altered sphingolipid metabolism.

Leukemia·2016

Same author

Relationship between progression-free survival and overall survival in chronic lymphocytic leukemia: a literature-based analysis.

Current oncology (Toronto, Ont.)·2015

Same author

Gefitinib targets ZAP-70-expressing chronic lymphocytic leukemia cells and inhibits B-cell receptor signaling.

Cell death & disease·2014

Area of Science:

Biochemistry
Materials Science
Analytical Chemistry

Background:

Hyaluronan is a crucial glycosaminoglycan in biological tissues and medical devices.
Accurate quantification of bound hyaluronan is essential for quality control and development.
Existing methods may be destructive or lack specificity.

Purpose of the Study:

To develop a simple, convenient, and nondestructive method for quantifying bound hyaluronan.
To adapt the method for assessing hyaluronan on medical device surfaces.

Main Methods:

Utilizes the cationic dye Toluidine Blue O, which binds to the D-glucuronate component of hyaluronan.
Quantification is achieved by measuring dye binding, avoiding interference from metachromatic properties.
The method is adaptable for assessing hyaluronan coatings on various substrates.

Main Results:

A straightforward, non-destructive assay for bound hyaluronan was established.
The method provides accurate quantification without interference from dye properties.
Successful adaptation for hyaluronan-coated medical devices demonstrated.

Conclusions:

The described method offers a reliable and accessible way to quantify bound hyaluronan.
This technique is particularly valuable for the development and manufacturing of hyaluronan-coated medical devices.
The method facilitates quality control and innovation in biomaterials.

Related Experiment Videos