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Related Experiment Videos

Apoptosis in dilated cardiomyopathy.

B K Hong1, H M Kwon, K H Byun

  • 1Cardiology Division, Yonsei Cardiovascular Center, Yonsei University College of Medicine, Seoul, Korea.

The Korean Journal of Internal Medicine
|March 14, 2000
PubMed
Summary

Dilated cardiomyopathy (DCMP) involves myocyte loss, potentially due to apoptosis. This study confirms that programmed cell death, not necrosis or inflammation, is the primary mechanism for cell loss in DCMP.

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Area of Science:

  • Cardiovascular Pathology
  • Cell Biology
  • Molecular Cardiology

Background:

  • Dilated cardiomyopathy (DCMP) presents with myocardial fibrosis and hypertrophy.
  • Cellular degeneration, not necrosis, is observed in cardiac hypertrophy.
  • The mechanism of myocyte loss in DCMP remains unclear.

Purpose of the Study:

  • To investigate if apoptosis is the cause of myocyte loss in DCMP.
  • To differentiate apoptosis from necrosis and inflammation in DCMP.

Main Methods:

  • Endomyocardial biopsies from DCMP patients and controls were analyzed.
  • Immunohistochemistry (TdT-mediated dUTP-biotin nick end-labeling) identified apoptosis.
  • Confocal laser scanning microscopy confirmed apoptotic morphology.

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Main Results:

  • Apoptosis was extensively observed in myocytes (19.8-25.4% apoptotic index).
  • Apoptosis was also detected in interstitial and vascular endothelial cells.
  • Morphological analysis revealed characteristic condensed chromatin indicative of apoptosis.

Conclusions:

  • Myocyte loss in DCMP is primarily caused by apoptosis.
  • Apoptosis affects both myocytes and interstitial cells in DCMP.
  • Inflammation and necrosis are not the main drivers of cell loss in DCMP.