Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ly6d-L, a cell surface ligand for mouse Ly6d.

J Apostolopoulos1, I F McKenzie, M S Sandrin

  • 1Molecular Immunogenetics and Transplantation Laboratory, Austin Research Institute, Austin and Repatriation Medical Centre, Heidelberg, Victoria, Australia. j.apostolopoulos@ari.unimelb.edu.au

Immunity
|March 14, 2000
PubMed
Summary

Researchers identified a novel ligand for the mouse Ly6d protein (ThB) on lymphocytes. This Ly6d ligand (Ly6d-L) is a small, cysteine-rich protein crucial for understanding lymphocyte interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fucosyl transferase (H) transgenic heart transplants to Gal-/- mice.

Transplantation·2000
Same author

Human monocytes bind to ligands on porcine endothelium containing the gal alpha (1,3)-Gal antigen.

Transplantation proceedings·2000
Same author

Immunotherapy with mannan-MUC1 and IL-12 in MUC1 transgenic mice.

Vaccine·2000
Same author

Aldehyde-mannan antigen complexes target the MHC class I antigen-presentation pathway.

European journal of immunology·2000
Same author

A role for IL-5 in the induction of cytotoxic T lymphocytes in vivo.

European journal of immunology·2000
Same author

The immune response of mice and cynomolgus monkeys to macaque mucin 1-mannan.

Vaccine·2000

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • The mouse Ly6 gene family encodes cell surface proteins involved in lymphocyte activation.
  • Ligands for Ly6 proteins, particularly Ly6d (ThB), have not been previously identified.
  • Understanding these interactions is key to deciphering immune cell signaling.

Purpose of the Study:

  • To identify and characterize the ligand for the mouse Ly6d protein (ThB).
  • To elucidate the molecular nature and cellular distribution of the Ly6d ligand.
  • To explore potential functional roles and structural homologies of the identified ligand.

Main Methods:

  • Utilized microspheres coated with Ly6d for affinity purification.
  • Screened a mouse spleen/thymus cDNA library using a panning technique against Ly6d.

Related Experiment Videos

  • Characterized the isolated ligand through biochemical and structural analyses.

    • Main Results:

    • Successfully identified a novel ligand for Ly6d, termed Ly6d-L.
    • Ly6d-L is a 9 kDa, nonglycosylated, cysteine-rich protein lacking a transmembrane domain.
    • Ly6d-L exhibits broad cellular distribution and associates with a 30 kDa protein on the cell surface.
    • Ly6d-L shows homology to a Notch EGF repeat.

    Conclusions:

    • The identification of Ly6d-L provides the first known ligand for Ly6d, advancing the understanding of Ly6 protein interactions.
    • Ly6d-L's unique structure suggests novel mechanisms for cell surface signaling.
    • Further research into Ly6d-L function may reveal new pathways in lymphocyte biology and immune responses.