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Related Experiment Video

Updated: Jul 26, 2026

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

Ly6d-L, a cell surface ligand for mouse Ly6d.

J Apostolopoulos¹, I F McKenzie, M S Sandrin

¹Molecular Immunogenetics and Transplantation Laboratory, Austin Research Institute, Austin and Repatriation Medical Centre, Heidelberg, Victoria, Australia. j.apostolopoulos@ari.unimelb.edu.au

|March 14, 2000

Summary

Researchers identified a novel ligand for the mouse Ly6d protein (ThB) on lymphocytes. This Ly6d ligand (Ly6d-L) is a small, cysteine-rich protein crucial for understanding lymphocyte interactions.

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Murine Dermal Lymphatic Endothelial Cell Isolation

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Published on: July 21, 2023

Area of Science:

Immunology
Molecular Biology
Cell Biology

Background:

The mouse Ly6 gene family encodes cell surface proteins involved in lymphocyte activation.
Ligands for Ly6 proteins, particularly Ly6d (ThB), have not been previously identified.
Understanding these interactions is key to deciphering immune cell signaling.

Purpose of the Study:

To identify and characterize the ligand for the mouse Ly6d protein (ThB).
To elucidate the molecular nature and cellular distribution of the Ly6d ligand.
To explore potential functional roles and structural homologies of the identified ligand.

Main Methods:

Utilized microspheres coated with Ly6d for affinity purification.
Screened a mouse spleen/thymus cDNA library using a panning technique against Ly6d.
Characterized the isolated ligand through biochemical and structural analyses.

Main Results:

Successfully identified a novel ligand for Ly6d, termed Ly6d-L.
Ly6d-L is a 9 kDa, nonglycosylated, cysteine-rich protein lacking a transmembrane domain.
Ly6d-L exhibits broad cellular distribution and associates with a 30 kDa protein on the cell surface.
Ly6d-L shows homology to a Notch EGF repeat.

Conclusions:

The identification of Ly6d-L provides the first known ligand for Ly6d, advancing the understanding of Ly6 protein interactions.
Ly6d-L's unique structure suggests novel mechanisms for cell surface signaling.
Further research into Ly6d-L function may reveal new pathways in lymphocyte biology and immune responses.