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Behaviour of leukocyte acid phosphatase in various chronic diseases
Insights
Leukocyte acid phosphatase activity varied across chronic diseases. Myeloma patients showed markedly increased enzyme activity, while rheumatoid arthritis and hepatitis patients had moderate increases, suggesting potential links to immunoglobulin production.
Area of Science:
- Biochemistry
- Hematology
- Immunology
Background:
- Leukocyte acid phosphatases play roles in cellular functions.
- Chronic diseases can alter enzyme activity in leukocytes.
- Understanding these alterations can provide diagnostic or prognostic insights.
Purpose of the Study:
- To investigate leukocyte acid phosphatase levels in patients with various chronic diseases.
- To correlate enzyme activity with disease severity and immunoglobulin production.
Main Methods:
- Investigated leukocyte acid phosphatases in 146 patients with chronic diseases.
- Utilized a modified Kaplow and Burstone method, adjusting the incubation medium's pH.
- Quantified enzyme activity in granulocytes and lymphocytes.
Main Results:
- Normal to slightly increased acid phosphatase in chronic myeloid leukemia granulocytes.
- Moderate lymphocyte acid phosphatase activity in rheumatoid arthritis, chronic hepatitis, lupus erythematosus disseminatus, and chronic lymphocytic leukemia.
- Markedly increased enzyme activity observed in myeloma patients.
Conclusions:
- Leukocyte acid phosphatase activity differs significantly across chronic diseases.
- Myeloma exhibits a pronounced increase in enzyme activity.
- A potential correlation between enzyme activity intensity and immunoglobulin formation is suggested.
Abstract:
Leukocyte acid phosphatases were investigated in 146 patients with different chronic diseases. The method of investigation used was that of Kaplow and Burstone slightly modified by the authors in what regards the pH of the incubation medium. Normal or slightly increased scores were observed in the granulocytic series of patients with chronic myeloid leukemia. In patients with rheumatoid arthritis, chronic hepatitis, lupus erythematosus disseminatus and chronic lymphocytic leukemia a moderate enzymatic activity was generally observed in the lymphocyte and more marked in severe forms of disease. A marked increase of the enzyme activity was observed in patients with myeloma. The possibility of a correlation between the intensity of enzyme activity and immunoglobulin formation is discussed.