Related Experiment Video
Updated: Oct 17, 2026

Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
The human growth hormone receptor gene - characterisation of the liver-specific promoter
1Division of Medicine, Bristol Royal Infirmary, University of Bristol, Marlborough Street, Bristol, UK.
Abstract:
Several variants (V1-V8) have been described for the 5' untranslated region of human growth hormone receptor cDNA (Pekhletsky, R.I., Chernov B.K., Rubtsov, P.M., 1992. Variants of the 5'-untranslated sequence of human growth hormone receptor mRNA. Mol. Cell. Endocrinol. 90, 103-109). Transcription of one of these, variant V1, is under the control of a liver-specific promoter (Zou, L., Burmeister L.A., Sperling, M.A., 1997. Isolation of a liver-specific promoter for human growth hormone receptor gene. Endocrinology 138, 1771-1774). Further characterisation of this promoter shows that: (1) a cluster of exon 1 variants, which includes that coding for V1, is located about 13 kb upstream of the first coding exon; (2) the human V1 promoter (and not V7, as previously suggested) is homologous to liver-specific regulatory regions of rat and mouse GH receptor genes; (3) the transcription start site for V1 is 27 bp further upstream than previously reported; (4) a 158-bp sequence is sufficient for basal promoter activity, while larger constructs provide evidence for negative elements further upstream; and (5) nuclear proteins from HepG2 hepatoma cells protect regions of the V1 promoter from DNase digestion, revealing putative regulatory sites.
Related Concept Videos
Cell Specific Gene Expression
Liver Regeneration
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...
The Eukaryotic Promoter Region
The Eukaryotic Promoter Region
Reporter Genes
Commonly used reporter...
General Transcription Factors
