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[Effects of MMP-1 expressing plasmid on rat liver fibrosis]
1Institute of Infectious Disease, Xiangya Hospital, Hunan Medical University, Changsha.
Objective:
To observe the effects of MMP-1(Matrix Metalloproteinase-1) expressing plasmid on rat liver fibrosis.
Methods:
We constructed the rat's MMP-1 recombinant plasmid which can be expressed in eucaryotic cells. After encapsulating the MMP-1 recombinant plasmid by lipofectin, we transferred it to the rat's fibrotic liver in vivo intraperitoneally, then observed the effects of the plasmid on the fibrotic liver by RT-PCR, immunohistochemistry and the observation of pathology.
Results:
The recombinant plasmid of MMP-1 could increase the degradation of type I and type III collagen in the rat's fibrotic liver according to immunohistochemistry compared with fibrotic modal group(P < 0.01) or compared with colchicine group(P < 0.05). According to the observation of pathology, recombinant plasmid of MMP-1 has some reverse effects on the rat's fibrotic liver compared with fibrotic model group(P < 0.05), but no obvious difference was found between the plasmid and colchicine(P > 0.05).
Conclusion:
The recombinant plasmid of MMP-1 has some reverse effects on liver fibrosis, but the effects is limited.