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Related Experiment Videos

Fission yeast switches mating type by a replication-recombination coupled process.

B Arcangioli1, R de Lahondès

  • 1Unite des Virus Oncogenes, URA 1644 du CNRS, Departement des Biotechnologies, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, Cedex 15, France.

The EMBO Journal
|March 16, 2000
PubMed
Summary
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Fission yeast mating-type switching involves a novel DNA intermediate during S-phase. This discovery supports a replication-recombination pathway for mating-type switching in Schizosaccharomyces pombe.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Fission yeast (Schizosaccharomyces pombe) has a homothallic life cycle with regulated mitotic mating-type alternation.
  • Mating-type switching in one of two sister cells is a key feature of this life cycle.
  • Previous research identified site- and strand-specific DNA modification at the mat1 locus preceding switching.

Purpose of the Study:

  • To investigate the molecular mechanism and timing of mating-type switching in fission yeast.
  • To identify and characterize any DNA intermediates involved in the mating-type switching process.
  • To provide experimental evidence for proposed models of mating-type switching.

Main Methods:

  • Tracking the fate of mat1 DNA throughout the cell cycle.

Related Experiment Videos

  • Utilizing a Polymerase Chain Reaction (PCR) assay to detect DNA intermediates.
  • Analyzing the timing and rate of appearance and disappearance of the identified DNA intermediate.

    • Main Results:

    • A novel DNA intermediate of mating-type switching was identified during the S-phase of the cell cycle.
    • The temporal dynamics of this intermediate align with a replication-recombination coupled pathway.
    • Experimental evidence supports a copy choice recombination model for mating-type switching in Schizosaccharomyces pombe.

    Conclusions:

    • Mating-type switching in fission yeast occurs via a replication-recombination coupled pathway.
    • The identified S-phase DNA intermediate is crucial for this process.
    • The mechanism is analogous to sister chromatid recombination observed during DNA damage repair.