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Updated: Aug 1, 2026

A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
Published on: August 13, 2009
Immunocytochemical techniques to investigate the pathogenesis of infectious micro-organisms and the concurrent immune
S H Jeurissen1, E Claassen, A G Boonstra-Blom
1Institute for Animal Science and Health ID-DLO, Department of Immunology, Lelystad, The Netherlands. s.h.m.jeurissen@id.wag-ur.nl
Abstract:
For poultry as well as for mammalian species used for scientific research, many immunocytochemical techniques have been developed to investigate in detail the interaction between infectious micro-organisms and the nonspecific and specific immune systems of the host. In this review three techniques have been described with all technical details necessary to perform them correctly: (1) single immunocytochemical staining to detect the infectious micro-organisms in situ at their site of infection, (2) double immunocytochemical staining to visualize simultaneously the infectious micro-organism and the host cellular response to investigate their interactions, and (3) detection of plasma cells producing antibodies specific to the micro-organism. Of the three techniques the results are described when applied on chicken tissues infected with various micro-organisms, such as Marek's disease virus, chicken anemia virus, infectious bursal disease virus and Eimeria tenella.
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