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Related Experiment Videos

Eel WT1 sequence and expression in spontaneous nephroblastomas in Japanese eel.

Y Nakatsuru1, K Minami, A Yoshikawa

  • 1Department of Pathology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.

Gene
|March 16, 2000
PubMed
Summary

The Wilms' tumor 1 gene (WT1) plays a role in eel kidney tumor development. Researchers found aberrant WT1 expression in eel nephroblastomas, suggesting its involvement in tumor genesis.

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Area of Science:

  • Comparative oncology
  • Molecular biology
  • Genetics

Background:

  • Nephroblastomas, a type of kidney cancer, can spontaneously develop in Japanese eels.
  • The Wilms' tumor 1 gene (WT1) is crucial in mammalian kidney development and tumorigenesis.

Purpose of the Study:

  • To investigate the role of the WT1 gene in spontaneous nephroblastomas in Japanese eels.
  • To understand the function of the eel WT1 counterpart, EWT1, in kidney tumor development.

Main Methods:

  • Cloning and analysis of the eel WT1 counterpart (EWT1).
  • Gene expression analysis using in situ hybridization.
  • Mutation and polymorphism analysis of the EWT1 gene in eel tumors.

Main Results:

Related Experiment Videos

  • The eel EWT1 gene shares conserved features with mammalian WT1, including a splice site and zinc finger domain, but lacks specific transregulator regions.
  • EWT1 is expressed in eel kidney, testis, and spleen, with positive signals in immature kidney cells.
  • No mutations were found in EWT1 in eel nephroblastomas, but polymorphic changes were observed.
  • Aberrant WT1 expression occurred in 44% of epithelial and 60% of nephroblastic tumor cells.
  • EWT1-expressing cells in eel tumors resemble human Wilms' tumor blastema cells.

Conclusions:

  • The EWT1 protein is likely involved in normal eel kidney development.
  • Aberrant EWT1 expression suggests a role in the genesis of eel nephroblastomas, similar to WT1's role in mammals.