Related Experiment Videos
Intensity modulated HSQC and HMQC: two simple methods to measure 3J(HNH)alpha in proteins
P Permi1, I Kilpeläinen, A Annila
1Institute of Biotechnology, University of Helsinki, Finland.
Journal of Biomolecular NMR
|March 16, 2000
Abstract:
Two methods for the measurement of homonuclear 3J(HNH)alpha coupling constants are described. Both HSQC- and HMQC-type experiments employ 'quantitative J-correlation', in which the coupling constant of interest is obtained from the intensity ratio of cross peaks of two spectra. The first spectrum is acquired with 3J(HNH)alpha evolution and the second with alpha-proton decoupling. The resolution of these methods in the F1-domain is not restricted.