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Orexin-induced food intake involves neuropeptide Y pathway
1Department of Pharmacology, Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Brain Research
|March 17, 2000
Summary
Orexin-A stimulates feeding behavior, partly through the neuropeptide Y (NPY) pathway. Blocking NPY-Y1 receptors partially reduced orexin-A
Area of Science:
- Neuroscience
- Endocrinology
- Behavioral Science
Background:
- Orexins (orexin-A and -B) are neuropeptides regulating feeding behavior.
- Neuropeptide Y (NPY) is also implicated in appetite control.
Purpose of the Study:
- To investigate the role of NPY in orexin-A-induced feeding behavior.
- To determine if NPY-Y1 receptors mediate orexin-A's effects on appetite.
Main Methods:
- Administered orexin-A intracerebroventricularly to rats to induce feeding.
- Used BIBO3304, a specific NPY-Y1 receptor antagonist, to block NPY signaling.
- Measured food intake and analyzed Fos expression in NPY-containing neurons.
Main Results:
- Orexin-A significantly increased food intake in rats.
- Pre-administration of BIBO3304 partially inhibited orexin-A-induced feeding.
- Fos expression increased in NPY neurons after orexin-A administration, indicating NPY pathway activation.
- A negative control (BIBO3457) did not affect orexin-A's feeding effects.
Conclusions:
- Orexin-A partially mediates feeding behavior via the NPY pathway.
- The NPY system is one of the downstream pathways for orexin-A's appetite regulation.
- Additional pathways likely contribute to orexin-A-induced feeding behavior.