Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Carbohydrate-mediated recognition systems in innate immunity.

T Feizi1

  • 1Glycosciences Laboratory, Imperial College School of Medicine, Harrow, Middlesex, UK. t.feizi@ic.ac.uk

Immunological Reviews
|March 17, 2000
PubMed
Summary

Neoglycolipid technology enables identification of carbohydrate ligands for C-type lectin receptors, revealing cross-reactivity and potential for specificity. This advances understanding of innate immunity and cell recognition.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis.

Nature communications·2019
Same author

Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM.

Data in brief·2016
Same author

The First Total Synthesis of 6-Sulfo-de-N-acetylsialyl Lewis(x) Ganglioside: A Superior Ligand for Human L-Selectin.

Angewandte Chemie (International ed. in English)·2014
Same author

'Glyco-epitope' assignments for the selectins: advances enabled by the neoglycolipid (NGL) technology in conjunction with synthetic carbohydrate chemistry.

Advances in experimental medicine and biology·2003
Same author

New structural insights into lectin-type proteins of the immune system.

Current opinion in structural biology·2002
Same author

A monoclonal antibody, MIN/3/60, that recognizes the sulpho-Lewis(x) and sulpho-Lewis(a) sequences detects a sub-population of epithelial glycans in the crypts of human colonic epithelium.

Hybridoma·2001

Area of Science:

  • Immunology
  • Glycobiology
  • Cell Biology

Background:

  • Carbohydrate-recognizing receptors, including C-type lectins, are crucial for innate immunity, binding microbial or endogenous carbohydrates.
  • Identifying specific oligosaccharide ligands for these receptors is challenging due to heterogeneity and limited availability of samples.
  • C-type lectin-like proteins on NK and NKT cells regulate effector functions, with ligand identity remaining an open question.

Purpose of the Study:

  • To develop a microprocedure for direct binding studies of oligosaccharides derived from glycoproteins.
  • To identify novel oligosaccharide ligands for C-type lectin receptors, specifically selectins and collectins.
  • To explore the specificity and cross-reactivity of carbohydrate-receptor interactions.

Main Methods:

Related Experiment Videos

  • Introduction of neoglycolipid technology to create solid-phase oligosaccharide probes.
  • Direct binding experiments using these novel probes to identify receptor ligands.
  • Analysis of ligand specificity and cross-reactivity across different C-type lectin systems.

Main Results:

  • Neoglycolipid technology successfully generated oligosaccharide probes for binding studies.
  • Identification of previously unsuspected oligosaccharide ligands for selectins and collectins.
  • Observed significant cross-reactivity among identified ligands for different receptor systems.

Conclusions:

  • Neoglycolipid technology is effective for identifying carbohydrate ligands of C-type lectins.
  • Ligand specificity can be achieved through presentation and regulated receptor expression.
  • Further research is needed to determine if carbohydrates are ligands for NK and NKT cell C-type lectin-like receptors.