Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sialic acid and crystal binding.

C F Verkoelen1, B G van der Boom, D J Kok

  • 1Erasmus Urological Stone Treatment and Research (Eurostarr), Erasmus University, Rotterdam, The Netherlands. Verkoelen@uro.fgg.eurnl

Kidney International
|March 18, 2000
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Timelines of the "free-particle" and "fixed-particle" models of stone-formation: theoretical and experimental investigations.

Urolithiasis·2016
Same author

Calcium Oxalate Stone Agglomeration Inhibition [tm] Reflects Renal Stone-Forming Activity.

Ochsner journal·2011
Same author

Efficacy, safety and palatability of a new broad-spectrum anthelmintic formulation in dogs.

The Veterinary record·2011
Same author

The efficacy of milbemycin oxime against pre-adult Spirocerca lupi in experimentally infected dogs.

Veterinary parasitology·2010
Same author

The use of milbemycin oxime in a prophylactic anthelmintic programme to protect puppies, raised in an endemic area, against infection with Spirocerca lupi.

Veterinary parasitology·2010
Same author

Fatherhood in tall men treated with high-dose sex steroids during adolescence.

The Journal of clinical endocrinology and metabolism·2010

Cell surface sialic acid plays a key role in calcium oxalate monohydrate crystal adherence to kidney cells. Sialic acid presentation on immature cells facilitates crystal binding, suggesting its importance in kidney stone formation.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Nephrology

Background:

  • Investigated the role of cell surface sialic acid in calcium oxalate monohydrate (COM) crystal adherence to Madin-Darby canine kidney (MDCK) cells.
  • Focused on undifferentiated (crystal-binding) and maturated (noncrystal-binding) MDCK cells.

Purpose of the Study:

  • To elucidate the function of cell surface sialic acid in COM crystal adhesion to kidney cells.
  • To understand how sialic acid expression changes during epithelial cell maturation and its impact on crystal binding.

Main Methods:

  • Utilized lectins (MALII, SNA, PNA) to analyze cell surface oligosaccharides during epithelial development.
  • Assessed the effect of neuraminidase treatment on COM crystal binding using [14C]COM crystals.
  • Monitored sialic acid release via metabolic labeling with [3H]glucosamine.

Related Experiment Videos

Main Results:

  • Sialic acids, linked via alpha 2,3 and alpha 2,6 bonds, are expressed at different stages of MDCK cell development.
  • Neuraminidase treatment significantly reduced COM crystal binding to immature cells.
  • Mature cells exhibit higher sialylation, and PNA binding indicates sialic acid presence on terminal galactose units, which is revealed by neuraminidase treatment in mature cells.

Conclusions:

  • Sialic acids on immature cells may be oriented to favor COM crystal interactions.
  • Alternatively, sialic acids might facilitate the exposure of other crystal-binding molecules on the cell surface.