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Updated: Aug 10, 2026
11:19
Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets
Published on: July 8, 2010
Structural analysis of the gene encoding RP58, a sequence-specific transrepressor associated with heterochromatin
1Department of Immunology, National Institute of Infectious Diseases, Tokyo, Japan.
Gene
|March 18, 2000
Summary
The human rp58 gene, a transcriptional repressor in the brain, is structurally similar to its mouse counterpart. Its gene location on chromosome 1q44 ter is linked to neurological disorders and malignancies.
Area of Science:
- Molecular Biology
- Genetics
- Neuroscience
Background:
- RP58 is a sequence-specific transcriptional repressor with homology to zinc-finger proteins.
- It is highly synthesized in the brain and localized to condensed chromatin, suggesting a role in central nervous system transcriptional repression.
Purpose of the Study:
- To determine the complete genomic organization of the human rp58 gene.
- To compare the genomic structure of human and mouse rp58 genes.
- To investigate alternative splicing and chromosomal localization of the human rp58 gene.
Main Methods:
- Isolation of human rp58 gene genomic clones.
- Sequence analysis of human and mouse rp58 genes.
- RT-PCR analysis for alternative splicing.
- Fluorescence in situ hybridization (FISH) for chromosomal mapping.
Main Results:
- The human rp58 gene is uninterrupted over 4.2 kb.
- Human and mouse rp58 genes show high homology in protein sequences and genomic structure.
- An alternatively spliced form of rp58, similar to C2H2-171, was identified.
- The rp58 gene was localized to human chromosome 1q44 ter.
Conclusions:
- The human rp58 gene exhibits significant structural conservation with its mouse ortholog.
- Alternative splicing of rp58 occurs, producing variants like C2H2-171.
- The localization of rp58 to chromosome 1q44 ter links it to neurological disorders and malignancies, warranting further investigation.
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