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Related Experiment Videos

Intermolecular V(D)J recombination.

A Tevelev1, D G Schatz

  • 1Howard Hughes Medical Institute, Section of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520-8011, USA.

The Journal of Biological Chemistry
|March 18, 2000
PubMed
Summary
This summary is machine-generated.

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V(D)J recombination can occur between separate DNA molecules, forming coding joints. Linearization of substrates unexpectedly reduces coding joint formation, suggesting a "release and recapture" mechanism.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • V(D)J recombination is crucial for generating diverse antigen receptors in B and T lymphocytes.
  • This process is implicated in chromosomal translocations, potentially via interchromosomal recombination.

Purpose of the Study:

  • To investigate the V(D)J recombination machinery's capacity for intermolecular recombination.
  • To explore the formation of coding joints in trans using plasmid substrates.

Main Methods:

  • Investigated intermolecular V(D)J recombination between unlinked and catenated plasmids.
  • Assessed the impact of substrate linearization on coding joint formation.

Main Results:

  • V(D)J recombination occurred in trans between plasmid substrates, forming signal and coding joints.

Related Experiment Videos

  • Substrate linearization significantly reduced coding joint formation without altering cis/trans status.
  • No mechanistic block to trans coding joint formation was observed.

    • Conclusions:

    • Proposed a "release and recapture" model for V(D)J recombination.
    • Coding ends are frequently released from post-cleavage complexes and recaptured for joint formation.
    • Chromosomal V(D)J recombination likely involves mechanisms preventing end release or facilitating recapture.